POS-FRI-069

Protein handling and assay development through the use of polymeric metal chelation coupling

P Vukovic, C McElnea, Y Gao, L Yang, T Ling, A Wong, BT Ohse, R de las Heras, NJ Maeji and CY Huang

Anteo Technologies Ltd., Eight Mile Plains, Brisbane, Australia

Immobilisation of proteins onto solid supports is a necessary element of many applications used in protein science. Traditionally, proteins are adsorbed onto surfaces by passive means or by a variety of covalent chemistries that can adversely impact both the structure and function of the immobilised protein. New generation applications, solid-phase surfaces and miniaturisation, together with increasingly complex assay platforms continue to challenge the conventional methods used for protein surface attachment. Anteo Technologies has developed an alternative approach that utilizes a metal nanopolymer, chelation-based surface chemistry. This approach relies on cationic metal polymers in aqueous solution that bind to synthetic surfaces with electron donating potential, resulting in the formation of nanometer thin coatings that are very strong and stable. The polymeric metal ions chelate and bind by multiple avidity points to both the surface and to biomolecules, thereby acting as a molecular binder. We demonstrate the technology’s versatility and effectiveness to simply and gently bind proteins onto most of the solid support surfaces typically used in ELISAs, antibody coupled bead based assays, lateral flow assays, bioprocessing and biosensor applications for a broad spectrum of protein science applications.