Australian Institute for Bioengineering & Nanotechnology, The University of Queensland, st Lucia, QLD 4072 Australia
Molecular Cloning Designer Simulator (MCDS) is a powerful new all-in-one cloning and genetic engineering design, simulation and management software platform developed for complex synthetic biology and metabolic engineering projects. In addition to standard functions, it has a number of features that are either unique, or are not found in combination in any one software package: (1) It has a novel interactive flow-chart user interface for complex multi-step processes, allowing an integrated overview of the whole project; (2) It can perform a user-defined workflow of cloning steps in a single execution of the software; (3) It can handle multiple types of genetic recombineering, a technique that is rapidly replacing classical cloning for many applications; (4) it includes experimental information to conveniently guide wet lab work; and (5) it can store results and comments to allow the tracking and management of the whole project in one platform. t is particularly useful for management of complex synthetic biology and metabolic engineering projects. MCDS is freely available from https://mcds.codeplex.com/.